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Adequate concentrations of dissolved oxygen in streamflow are necessary for fish life and other aquatic organisms and the prevention of offensive odors. Dissolved oxygen levels are considered the most important and commonly employed measurement of water quality and an indicator of a water body's ability to support desirable aquatic life. Monitoring data developed by the State of Georgia show widespread violations of water quality standards in the Suwannee River Basin due to low dissolved oxygen. The contributing factors are poorly understood. Relationships between stream flow and dissolved oxygen for the Little River Watershed near Tifton, Georgia were examined. Data collected by the Southeast Watershed Research Laboratory and the University of Georgia indicate that low flow conditions may lead to low dissolved oxygen levels. These data are critical for determining background conditions and for determining the factors controlling oxygen depletion in streams throughout the U.S. The data will assist in the development of effective land management programs for improving and maintaining stream water quality.