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A Spreeta SPR biosensor was evaluated as a diagnostic tool for detecting Salmonella spp. and E. coli O157:H7 in samples obtained from swine farms and slaughterhouses. A VIDAS immunosensor was used as a standard for comparing the accuracy of results obtained from the SPR biosensor. Field samples were obtained from representative small and medium-sized pork production facilities and analyzed using both the SPR biosensor and the VIDAS immunosensor. Results of this study indicate that with further refinement the SPR biosensor could be useful as a pathogen monitoring system within the pork industry chain. The average SPR biosensor response was greater in magnitude for S. Typhimurium- and E. coli O157:H7-positive samples than the average response to negative samples. However, the difference was statistically significant for only one sample. With further modifications of the initiation and sampling protocols, the SPR biosensor has potential to increase its ability to detect target organisms in field-samples. The SPR biosensor shows promise to provide a complementary detection system to standard lab-based systems currently used in food safety.