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Evaluation of a Surface Plasmon Resonance Biosensor in the Detection of Salmonella spp.

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  Paper number  016035,  2001 ASAE Annual Meeting. (doi: 10.13031/2013.5540) @2001
Authors:   C.A. Meeusen, E.C. Alocilja, W. Osburn
Keywords:   Foodborne pathogens, Salmonella Typhimurium, Food safety, Biosensors, Surface plasmon resonance (SPR)

The Spreeta SPR biosensor was used to detect Salmonella enterica spp. Typhimurium. The selectivity of the SPR biosensor was assayed using a series of antibody concentrations and dilution series of the organism. Specificity of the SPR biosensor was demonstrated in pure and mixed cultures of S. Typhimurium. The optimum antibody concentration for use on the SPR biosensor was 300 mg/ml, a result comparable to other immunosensors. The detection limit of the SPR biosensor for S. Typhimurium in pure culture was 10 7 colony forming units (CFU)/ml. A pure culture of the pathogen could be detected after 5 1/2 hours of enrichment. The SPR biosensor was specific to S. Typhimurium in pure cultures at concentrations of 10 7 CFU/ml. In mixed cultures, the detection limit of the biosensor was 10 7 CFU/ml for the target organism, when the non-target bacterial concentration was 10 6 CFU/ml or less. Results demonstrated the potential of SPR biosensor for rapid and specific pathogen detection. By changing the antibody used in the preparation phase, it can be used to detect many different substrates, including other foodborne pathogens. With further studies and refinements, the SPR biosensor shows promise to provide a complementary detection system to standard lab-based systems currently used in food processing plants.

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