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In Vitro Evaluation of Antibacterial and Antioxidative Activity of Two Northern Alabama Hemp (Cannabis sativa L) varieties

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  2021 ASABE Annual International Virtual Meeting  2100956.(doi:10.13031/aim.202100956)
Authors:   Aaron L Dudley, Lamin M Kassama, Armitra Jackson-Davis, Ernst Cebert, Anwar Mohammed
Keywords:   Antimicrobial, Antioxidative, BioScreen C, CBD, Disc Diffusion, DPPH.

Abstract. The application of plant bio-actives in food packaging is a promising technology to minimize postharvest food spoilage due to their antioxidant and antibacterial properties, which provide some form of natural protection. One such candidate is industrial hemp (Cannabis sativa) which contains bioactive components. The objective of this study was to evaluate the antibacterial and antioxidative activities of two Northern Alabama hemp varieties Jinma 1 (J1-Fiber type), and Cherry Wine (CW-CBD type). We hypothesize that CW varieties will have significantly higher antioxidative and antimicrobial activity. In this study, botanical parts (Bud-B and Stem-St) of two northern Alabama hemp varieties were used. The extraction solvent used was ethanol (96%) (E). The antioxidant potential of the extracts was determined by total phenolic, total flavonoid, DPPH (2,2-diphenyl-1- picrylhydrazyl). Antibacterial activity against cocktails of enteric pathogens E. coli 0157:H7 and Listeria monocytogenese was determined using a BioScreen-C microtiter and disc diffusion assays. Results were expressed, in triplicate, as mean value ± SE (n=3). Statistical significance was evaluated by one-way ANOVA and P<0.05 were significant. Results showed the total inhibition of DPPH ranged from 27.35% (CW-B-E) extract to 65.22% (J1-St-E) extract. Disc diffusion testing against E. coli zone of inhibition ranged from 9.42±0.39mm (J1-St-E) to 10.78±0.55mm (CW-St-E), Listeria monocytogenes 11.36±0.58mm (J1-St-E) to 25.79±0.83mm (CW B-E). Overall CBD extracts had higher antioxidant and antimicrobial activity strongly suggesting their use in the development of the active film. The development of hemp-based active packaging will contribute immensely to food preservation and global food supply chain sustainability.

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