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Detection of salbutamol in fresh muscle based on solid SERS substrate

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  2021 ASABE Annual International Virtual Meeting  2100298.(doi:10.13031/aim.202100298)
Authors:   Wenlong Zou, Yankun Peng, Qinghui Guo, Deyong Yang, Kuanglin Chao
Keywords:   surface enhanced Raman spectroscopy; solid substrate; fast detection; salbutamol; muscle tissues

Abstract. In this research, the silver nanospheric self-assembled monolayer was transferred to the polydimethylsiloxane (PDMS) sheet by the Langmuir-Schaefer method to develop a fast detection method of SERS for salbutamol in muscle tissues. The SERS spectra were obtained using 785 nm exciting radiation with 180 mW laser power and 5 s exposure time. The SERS spectra of organic sub stances have severe fluorescence background, so it is crucial to remove fluorescence background from Raman signal first for subsequent signal analysis. In this study, Savitzky-Golay 5-point smoothing filter and the adaptive iteratively reweighted Penalized Least Squares (airPLS) correction method were used to remove the random noise and the fluorescence background for improving the accuracy of SERS results. We acquired the SERS spectra of salbutamol standard solution with different concentration from 0.01mg/L to 7 mg/L. The SERS signals intensities decreased when the concentrations decreased. It could be found that Raman peak of salbutamol at 1609 cm-1 was still quite clear even at low concentration of 0.1 mg/L, which could be used for monitoring the salbutamol levels. Regression model showed a good linear relationship (R2=0.939) between the intensity of characteristic spectral peak at 1609 cm-1 and concentration of salbutamol. The reproducibility of SERS detection is a very important parameter for SERS method. SERS signals of 20 groups of muscle tissue samples with salbutamol of the same concentration were measured to evaluate the reproducibility, and the relative standard deviation (RSD) of 20 parallel samples at 1253, 1489, 1609 cm-1 were 6.63%, 6.82%, 5.34%, which indicated good stability of the present method. Muscle tissues were prepared with salbutamol at the concentration of 0.01-7 mg/kg. The lowest detectable levels for salbutamol concentration is 0.5 mg/kg for muscle tissues. Therefore, after the pretreatment of spectra, we constructed the model based on characteristic spectral peak of 1609 cm-1 intensity and salbutamol concentration in muscle tissues, and the determination coefficient of its content and the predicted measured value is 0.936, the detection range is 0.5-7.0 mg/kg. The recovery rate of muscle tissues is 58.4%-75.1%. The results showed that using solid SERS substrate to detect β-agonists in fresh muscle tissue was feasible and had better stability.

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