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Abstract. Microfluidic technologies are widely applied in food safety, environmental monitoring and clinical diagnosis due to its low reagent consumption, small size, automation, etc. Among several currently available microfluidic technologies, digital microfluidics (DMF) chip has attracted more attention in recent years. Here, a programmable DMF chip platform coupled with a portable instrument was developed, which could be programmed to complete biochemical experiment. A simple fabrication process for the production of DMF chip was carried out, and it was low-cost and had lower requirements on the environment during manufacturing process compared with the traditional DMF chip fabricated by photolithography. The platform contains a host computer, slave computer, DMF chip, monitor platform and custom software. On this platform, a large range (5-300 μL) of droplet was operated by program. Combing transporting and merging operation some biochemical experiments are able to be conducted by preset program hopefully. To verify the effect of the chip, in this work, it combined with the impedance biosensor to successfully conduct the detection of E. coli. DH5a.