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Abstract. The production and use of biodiesel benefits the development of a sustainable energy source in terms of the environmental, economic and social aspects and it also brings the perspective of the reduction of diesel oil imports in Brazil. The combustion and exhaust emissions characteristics of a diesel engine are affected by the fuel spray and the atomization characteristics as well as by the properties of the fuel. The aim of this study was to evaluate the fuel consumption of a diesel engine fueled with, at different times, commercial mineral diesel and blends of this diesel with the macaw palm oil biodiesel in proportions equivalent to B20, B50, B80 and B100. In Brazil, during the period of this experiment, according to the law, commercial mineral diesel fuel was composed of 7% of biodiesel and 93% of diesel fuel. For the tests, an AGCO Sisu Power 420DS diesel engine of an A 950 Valtra tractor was used. The 1585 ISO ABNT standard was used for carrying out the tests. The tests were carried out with the engine at full load. The fuel consumption was measured by means of an Oval flow meter. The net calorific value was of 36,800 kJ.kg^-1 for the B100 macaw palm oil biodiesel and of 43,900 kJ.kg^-1 for the commercial diesel. At the PTO speed rotation of 540 rpm, the fuel consumption was of 13.5 L.h^-1 when using B100 macaw palm oil biodiesel and of 10.5 L.h^-1 when the B7 commercial diesel was used (increase of 28.6% in relation to B7). At the same rotation, when B20 was used the fuel consumption was of 10.8 l.h^-1 (increase of 2.8% in relation to B7).