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Abstract. Vegetable crops are very susceptible to damage from weed competition, with early season weeds emerging within 3 weeks after crop emergence a control priority to prevent significant yield loss. One strategy for rapid weed crop differentiation is to place a machine detectable marker on the crop, i.e. Crop signaling. In this study, Rhodamine B (Rh-B) was used as a systemic crop signaling compound in tomato plants due to its unique fluorescent properties. The photostability of Rh-B was assessed under full sun treatment. The results showed that Rh-B was photostable in tomato plants for 27 days after transplanting.