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Abstract. Microbial fuel cell (MFC) has the potential to generate bioelectricity and simultaneously can treat wastewater using exoelectrogens as biocatalyst. In this study, custom-made dual MFC chambers were fabricated and evaluated for generating electricity using sugarbeet processing wastewater (SBWW) as well as reducing some target pollutants during the process. Three dual-chamber MFCs with pretreated Nafion as cation exchange membrane (CEM) between chambers and carbon cloth as electron acceptor (anode and cathode) were used. Sugarbeet processing wastewater was collected from American Crystal, MN, USA, and inoculum was collected from Fargo wastewater treatment plant, Fargo, ND, USA. Five times diluted raw SBWW with 5% inoculum having chemical oxygen demand (COD) in the range of 919-952 mg/L were used as anolyte and monobasic potassium phosphate (50 mM) with a pH of approximately 7.2 was used as catholyte. A preliminary study with approximately 280 mL of anolyte, a maximum of 832mV electricity was generated by MFCs at 10^th day. Overall, at the end of 70 days, an average 246.86 mV electricity was produced irrespective of anolyte volume. Additionally, approximately 90% of COD reduction was achieved in the study. This study demonstrated that SBWW could be used as a substrate of MFC to generate electricity as well as to refine wastewater to a certain extent.