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Abstract. Nowadays, the main harvesting method for Lycium barbarum L. (L. barbarum) is the manual harvesting, but the efficiency of the manual harvesting is low and the cost of that is high. To achieve the high efficiency and low damage of the mechanical harvesting of L. barbarum, a vibrating and comb brushing harvesting method for L. barbarum was proposed. The high-efficiency harvesting comb brush and low-damage harvesting collecting box were designed. A vibrating and comb brushing L. barbarum harvester was designed and the performance experiment of harvester was performed. Three factors and three levels quadratic orthogonal revolving experiment based on response surface methodology was adopted to establish the mathematical model regarding the harvesting rate of ripe fruit, the harvesting rate of unripe fruit, and the damage rate of ripe fruit affected by the revolving speed of comb brush, the revolving speed of cam, and the style of comb brush. The optimized parameters were determined to be the revolving speed of comb brush of 64.52 r/min, the revolving speed of cam of 29.68 r/min, and style Ⅱ as the style of comb brush. Field experiment results showed that the harvesting rate of ripe fruit was 89.12 %, the harvesting rate of unripe fruit was 5.87 %, and the damage rate of ripe fruit was 6.24 %. It provides a design basis for the later research of harvesting method for L. barbarum.