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Abstract. The applicability of near-infrared (NIR) spectroscopy combined with chemometrics was explored in this study to develop rapid, low-cost and non-destructive spectroscopic methods for quantification of amylose in milled rice. The optimal results are concluded based on the comparison between the results of diffuse transmission, diffuse transmission attenuation, and diffuse transmission light compensation.The rice samples were scanned using near infrared transmittance spectrometry over the wavelengths of 900-1700 nm before the physical and chemical experiments. The PLS prediction results (R_pre ) for diffuse transmission, diffuse transmission attenuation and diffuse transmission light compensation were0.9049,0.9139,0.9577,respectively.The RMSEP (and T-test(p=0.05)) were 1.5654%(0.59), 1.5337%(0.64),1.4261 (-0.51),correspondingly. The use of diffuse transmission light compensation has proved to be feasible to predict amylose content, which also significantly improved the accuracy of the model.