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Distribution of Particulate Matter in Poultry Layer Houses and In Vitro PM2.5-induced Damage on Human Alveolar Epithelial Cells
Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org
Citation: 10th International Livestock Environment Symposium (ILES X) .(doi:10.13031/iles.18-081)
Authors: Dan Shen, Pengyuan Dai, Yansen Li, Chunmei Li
Keywords: A549 cell, cytotoxicity, inflammatory response, layer house, particulate matter.
Abstract. Layer houses are an important source of particulate matter (PM) emissions, and exert potentially adverse effect on worker and animal health. The objectives of this study were 1) to analyze the distribution of PM of different size fractions in an enclosed layer house, and 2) assess in vitro PM2.5 damage on human alveolar epithelial cell line (A549 cell), which cultured with culture flask. The detection was taken from 05:00 h to 21:00 h every 2 h for a 7-d continuous monitoring for PM concentrations. A549 cells were treated under the concentration at 6.25, 12.5, 25, 50, 100, 200, 400 μg ml-1 of PM2.5 at 3 h, 6 h, 12 h, and 24 h time-points, respectively. The concentrations of PM10 and TSP were significantly higher in the rear of the chicken house comparing with the forepart (P < 0.05). The concentrations of PM10 and TSP at the first feeding time at 07:00 h increased dramatically compared with those before turning on the lights at 05:00 h (P < 0.05). Airflow speed showed significant correlation with other measured microclimatic variables. The PM2.5 at 200 and 400 μg ml-1 significantly decreased the viability of A549 cell for 6-h exposures (P < 0.05), and at 50, 100, 200, 400 μg ml-1 for 12-h and 24-h exposures respectively (P < 0.05). The content of reactive oxygen species (ROS) and calcium ion (Ca2+) in A549 cell increased after treatment with PM2.5 on the concentration of 25 μg ml-1 at 12 h. The tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8) were significantly increased in group treated with PM2.5 at the concentration of 25 μg ml-1 and 50 μg ml-1, 12.5 μg ml-1, 6.25 μg ml-1 respectively. The distributions of PM were affected by ambient humidity, airflow speed, and chicken activity. The PM2.5 collected in chicken houses decreased the viability of A549 cell in time - and dose - dependent manner, and induce inflammatory reaction.
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