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Abstract. In recent years, terahertz time-domain spectroscopy (THz-TDS) has attracted an increasing number of researchers to investigate the far-infrared vibrational modes or picosecond dynamic characteristics of a wide range of materials, which has proved to be a promising non-destructive technology for both qualitative and quantitative measurements. Undesirably, the strong absorption of water will considerably weaken the THz signal, which limited further application and development of THz-TDS, especially in the field of agriculture and food. To overcome this difficulty, we proposed a method by combining multiple attenuated total reflection (MATR) and THz-TDS to realize high sensitivity detection of high-absorption aqueous solution. In this paper, we investigated the responses of deionized water, glucose aqueous solution and NaCl aqueous solution through MATR-assisted THz-TDS and analyzed their response mechanism in terahertz region. According to the results, the lowest concentration detected in glucose aqueous solution and NaCl aqueous solution were ~0.1 mg/mL and ~0.01 mg/mL, respectively, which were much lower than that of traditional transmission method, demonstrating higher sensitivity detection of high-absorption aqueous solution could be realized by MATR THz-TDS.