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Abstract. Green light during artificial incubation can affect the development of chick embryos. However, there has been relatively little work systematically assessing dose-effect relationships between green light and metabolism rate in chick embryos. Eggs (n=800) were incubated under photoperiods of either 0 h of light and 24 h of darkness (0L:24D), 6 h of light and 18 h of darkness (6L:18D), 12 h of light and 12 h of darkness (12L:12D), or 18 h of light and 6 h of darkness (18L:6D). In accordance with dose-effect on embryonic development, the response of carbon dioxide production rate to variations in illuminance was consistent with lipid reserve in yolk residue and hepatic glycogen in chick embryos. This sensitivity to green light indicates that the variation in illuminance within certain range of green light can have a significant impact on embryonic development and metabolic rate in chick embryos during artificial incubation. The results of this study indicate that providing 18 h of green light during incubation can have significantly improved the development and metabolism of the chick embryos.