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Inactivation Mechanism and Apoptotic-Like Changes in Aeromonas hydrophila Induced by Slightly Acidic Electrolyzed Water in Freshwater

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  Transactions of the ASABE. 61(1): 305-314. (doi: 10.13031/trans.12560) @2018
Authors:   Zhangying Ye, Shuo Wang, Weishan Gao, Songjian Nan, Songming Zhu, Jinsong He, Dezhao Liu
Keywords:   A. hydrophila, Antibiotics, Anti-infection, Cell apoptosis, Membrane permeability, Slightly acidic electrolyzed water.

Abstract. This study investigated the suppressive effect and the inactivation mechanism of slightly acidic electrolyzed water (SAEW) on . The experiment compared different inhibition effects with SAEW and two antibiotics, gentamicin and norfloxacin. The conditions of pH of 6.5, oxidation-reduction potential (ORP) of 890 mV, available chlorine concentration (ACC) of 28 mg L-1 of SAEW, and volume ratios of 1:10, 1:15, and 1:20 were used. Using exposure times of 5 and 10 min to analyze the inactivation mechanism of SAEW from the perspectives of cell membrane permeability, cell nuclear morphology, cell fluorescence staining, and other aspects, the anti-infection effects of SAEW were tested using tilapia () culture experiments. SAEW had a stronger bacteriostasis effect than the antibiotics used. SAEW can inhibit the ability to reproduce, causing abnormal cell morphology and cell elongation. The cell volume was increased with a change in the membrane permeability. The cell nuclear morphology was changed, further inducing apoptosis. Based on study of tilapia in a control group, the organs (intestine, stomach, and liver) developed different degrees of infection. No obvious infection was observed in muscle samples. After SAEW treatment, SAEW showed a better antibacterial effect and improvement effects on the inflammation and structural damage induced by , which could be helpful in aquaculture application.

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