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Abstract. A low-cost dye detection system was developed for pattern testing agricultural aircraft. The system uses a green laser pointer, a USB web camera outfitted with a standard rhodamine filter, and software to threshold the images. When arranged in a 90° illumination chamber, the system was able to detect spray patterns on strings that matched patterns derived from a standard WRK fluorometer system with a numerical comparison of 85% and a coefficient of variation of 17%. Visual assessment indicated an 86% match and all major peaks, valleys, and patterns were correctly indicated. A field test with the system indicated that correct airplane adjustments were possible with the system and advantages include that it is easy-to-build, composed of low-cost components, and the operator can see the string fluorescence during analysis. This system may provide an alternate dye detection system for pattern testing strings from agricultural aircraft.