Antihypertensive and antioxidant capacity of a high protein beverage (walnut-sesame seeds- oat-soybean)

Abstract. Cereals, legumes and oilseeds are characterized by a high protein value and for their potential to prevent cardiovascular diseases. This potential is attributed in part, to the presence of antioxidants and antihypertensive peptides. This study aimed to formulate and develop a drink based on walnut, oat, sesame seeds and soybean to evaluate in vitro its antihypertensive an antioxidant capacity. The beverage was formulated by the method of protein digestibility–corrected amino acid score. Subsequently, the total protein was extracted and hydrolyzed with trypsin. The hydrolysates were analyzed by 2D-PAGE and LC-MS/MS for the identification of bioactive peptides. The antihypertensive capacity was determined by the ACE-1 (Angiotensin converting enzyme) inhibition assay (using captopril as a control) and the antioxidant activity by the methods ORAC and DPPH. This beverage has a 100% protein quality; its protein content covers 48% of the RDA (Recommended Daily Intake). There was presence of antihypertensive (34.34%) and antioxidant (23.34%) peptides. It was observed that 600 μg/mL of hydrolysates inhibited 85% of the activity of ACE-1, while 500 μg/mL of the control inhibited 89.29%, showing significant difference (p<0.05). The ORAC method showed an antioxidant capacity of 98.65 μmolET/g in contrast with the 38.44 μmolET/g by DPPH. In conclusion, this drink is an excellent source of antioxidants and active peptides to prevent hypertension.