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Assessment of sterilization methods on by-products used in Total Mixed Ration for safe feed production
Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org
Citation: 2017 ASABE Annual International Meeting 1700877.(doi:10.13031/aim.201700877)
Authors: Daniel Dooyum Uyeh, Seung Min Woo, Jun Hee Kim, Yushin Ha
Keywords: By-products, Steam treatment, Sterilization, Total mixed ration, Ultra-violet radiation
Abstract.: Several studies focus on finding alternative feed for livestock farms to reduce cost in breeding. Formulating Total Mixed Ration (TMR) feed with agricultural by-products (mushroom, palm kernel expeller, soy sauce cake etc.) via fermentation using effective microbes as facilitators has been in consideration lately. Unwanted microbes usually contaminate and compete with the effective microbes intended to act on the by-products. Establishing an efficient fermentation protocol for a safe by-product feed is the research focus. Mixed by-products were collected from TMR plant in Gyeongju, South Korea using aseptic techniques. Initial analyses indicated the presence of Bacillus cereus (3.0 x 106 CFU/g), Yeast (less than 106 CFU/g) and Lactic Acid Bacteria (106 CFU/g) in the untreated samples. Different selected conditions of steam treatment (121°C, 100°C, and 80°C at 15 minutes respectively) and Ultra Violet (UV) radiation (320 nm for 15 minutes) were investigated. Enumeration for the pathogenic microbe (Bacillus cereus) after sterilization confirmed presence of less than 106 CFU/g at the different steam treatment conditions whereas UV treatment reduced the concentration to 106 CFU/g. Steam sterilization was more efficient and considered for inclusion in setting up a fermentation smart tank.