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A novel photoelectrochemical biosensor for measuring the droplets deposition

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  2016 ASABE Annual International Meeting  162460588.(doi:10.13031/aim.20162460588)
Authors:   Xiaoya Dong, Feng Wang, Xianping Guan, Mingxiong Ou, Baijing Qiu
Keywords:   Biosensor, chlorpyrifos, spray deposition, photoelectrochemical

Abstract. A novel strategy is presented for detection of droplets deposition using photoelectrochemical approach. An acetylcholinesterase(AChE) was immobilized on copper nanoparticles (Cu NPs) decorated nitrogen-doped graphene (N-G) modified glassy carbon electrode (GCE). A chlorpyrifos biosensor was fabricated using the AChE loaded on copper nanoparticles decorated nitrogen-doped grapheme modified glassy carbon electrode (AChE/Cu–N-G/GCE) as a working electrode, Ag/AgCl as standard and Pt wire as an auxiliary electrode connected through a photoelectrochemical equitment. AChE can hydrolyze acetylthiocholine chloride into thiocholine, which could produce the photocurrent of the enzyme electrode, and the further inhibition of chlorpyrifos on the enzyme electrode could decrease the photocurrent response. Based on the extent of AChE deactivation by the chlorpyrifos, a simple method for detection of chlorpyrifos in the droplet is proposed. Our research group has used this photoelectrochemical biosensor in spray application trials. Chlorpyrifos could be determined in the range of 0.001 ~ 1.0 μg mL−1 with a detection limit of 0.5 ng mL−1. The experiments showed that this method can be used to assess the distribution of spray application techniquesThe abstract is often the only part of the paper to be read, so include your major findings in a useful and concise manner. Include a problem statement, objectives, brief methods, quantitative results, and the significance of your findings. The abstract should be no more than 250 words long.

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