ASABE Technical Library - Abstract

Abstract. Electrospinning is the technique to fabricate nanofibers (NFs) using hydrostatic and electric forces. Recently it has gotten a spotlight because its structure is very similar with extracellular matrix (ECM). Particularly, aligned NFs were known for better regeneration ability than random NFs owing to its better similarity with native ECM. The graphene and its subfamily also improves osteogenesis, neurogenesis, epithelial differentiation, and cardiomyogenic differentiation of stem cells. If it is incorpotated in the aNFs, they would enhance the osteogenic differentiation. Thus, in this study, aligned reduced graphene oxide-incorporated NFs (RGO-NFs) were developed and the aligned RGO-NFs were applied on dental pulp stem cells (DPSCs) for their proliferation and osteogenesis. The alignments of the aligned RGO-NFs were confirmed by scanning electron microscopy. By chemical analysis, it was exhibited that the RGO was successfully incorporated in the RGO-NFs. The aligned RGO-NFs significantly increased the proliferation of the DPSCs at 10% compared to random RGO-NFs. The aligned RGO-NFs also induced the DPSCs to be well-aligned. In the osteogenic differentiation study, they enhanced the expression of osteocalcin and significantly improved calcium deposition. It is expected that the main reason of osteogenic capability of aligned RGO-NFs is the interaction between aligned NFs and RGO. In conclusion, the RGO-aNFs not only induced the alignment of the DPSCs but also significantly accelerated the osteogenic differentiation at 5%. The aligned RGO-NFs can be an efficient platform for osteogenic differentiation of the DPSCs, resulting in bone regeneration.