Click on “Download PDF” for the PDF version or on the title for the HTML version.
If you are not an ASABE member or if your employer has not arranged for access to the full-text, Click here for options.
A Portable Impedance Biosensing System for Rapid Detection of Avian Influenza Virus
Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org
Citation: Transactions of the ASABE. 59(2): 421-428. (doi: 10.13031/trans.59.11129) @2016
Authors: Zach Callaway, Yixiang Wang, Benhua Zhang, Tiemin Zhang, Thomas A. Costello, Michael F. Slavik, Yanbin Li
Keywords: Avian influenza virus, Biosensing system, Impedance measurement, Virtual instrument, Virus detection.
Abstract. Avian influenza virus (AIV) H5N1 is a highly infectious pathogen that can be dangerous to both poultry and humans. A rapid and sensitive detection method is needed to help prevent the spread of AIV H5N1. In this study, a portable impedance biosensing system was designed to integrate a laptop computer with LabVIEW software, a data acquisition (DAQ) device, a sample delivery system, a micro-flow cell, and a gold interdigitated microelectrode (IDME) into an automatic instrument for rapid detection of AIV H5N1. Streptavidin was first immobilized onto the IDME surface, and then biotin-labeled H5N1-specific aptamer was immobilized on the IDME surface through streptavidin and biotin binding. Samples were delivered through the sample delivery system into the micro-flow cell, and AIV H5N1 was captured by the aptamer on the IDME, resulting in a change in the impedance. A virtual instrument (VI) was programmed with LabVIEW software to provide a platform for sample delivery, impedance measurement, data processing, and control. The audio card of the laptop was used as a function generator, while the DAQ device was used for data communication. The impedance measured by this biosensing system was compared with that measured by a BAS IM6 impedance analyzer, and the error was less than 5%. The results indicated that the developed system could detect AIV H5N1 at a detection limit of 0.84 HAU per 200 μL without interaction with three non-target AIV subtypes (H1N1, H5N2, and H5N3). The system was portable and automatic, and it showed great potential as a diagnostic and epidemiological tool for detection of AIV in the field.
(Download PDF) (Export to EndNotes)