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The investigation of two stage cultivation on biomass, lipid content and FAME profiles composition capacity of microalgae: Desmodesmus sp. EJ 8-10 in anaerobic digestion wastewater

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  Paper number  141900295,  2014 Montreal, Quebec Canada July 13 – July 16, 2014. (doi: 10.13031/aim.20141900295) @2014
Authors:   L. Ning, A. Pang, F. Ji, G. Li, Y. Liu, Y. Zhou, R. Dong
Keywords:   Biomass, FAMEs, Lipid yield, Seeding size

Abstract. The microalgae, Desmodesmus sp., is considered to be a promising photoautotrophic biological material for biodiesel with high biomass productivity, lipid and FAME profiles accumulation accompanied by nitrogen and phosphorus removal. This investigation objective was to examine the effects of nutrient repletion on the biomass yield and lipid yield of Desmodesmus sp. in two cultivation stage for their potential capacity in biodiesel production. The results demonstrated that, in stage I, with the consumption of nutrients, the growth rate become slowly. Under the condition of 27°C, 6000 lux illumination, above 99 % and 100% of the ammonia and phosphorus had been removed after 14 and 7 days respectively. In stage II, two seeding size 0.8 g/L and 1.0 g/L of algal growth rate and lipid production were investigated and the highest lipid yield was 370 mg/L at the cultivation time of day 6 in stage II of size 1.0 g/L and related fatty acid methyl ester have been tested. Fatty acid are predominantly composed of palmitoleic (C16:0) and linoleic (C18:2n6c) about 38.20% which are suitable for biodiesel production.

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