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Design of a Vacuum Flashing System for High-Solids Fermentation of Corn
Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org
Citation: Transactions of the ASABE. 56(6): 1441-1447. (doi: 10.13031/trans.56.10277) @2013
Authors: Jameel Shihadeh, Haibo Huang, Kent Rausch, Mike Tumbleson, Vijay Singh
Keywords: Corn ethanol, Enzyme, High-solids fermentation, Vacuum flash.
Abstract. Efficiency gains in current grain ethanol processes are limited by limitations of the yeast biocatalyst. Yeast stress, including glucose concentrations (15% w/v) produced during liquefaction and saccharification and subsequent high ethanol concentrations (18% v/v) produced during fermentation, restrict slurry solids to 32% w/w for grain ethanol processes. A system was constructed to circumvent this solids limitation by combining two technologies: (1) granular starch hydrolyzing enzyme (GSHE), which can liquefy starch simultaneously with fermentation, and (2) reduced vapor pressure to remove ethanol from high-solids fermentations. GSHE eliminates the need for a separate liquefaction step because it gradually digests raw starch to glucose, which results in lower initial glucose concentrations (5% w/w). Vacuum was applied to remove ethanol as concentrations increased to near inhibition levels. An in situ ethanol removal system was constructed to conduct fermentation at 40% solid content. The vacuum flashing process successfully removed ethanol from the fermentation broth, thereby maintaining ethanol concentration in the broth below 10% to 12% v/v, while ethanol concentration in the control experiment without vacuum stripping was above 18% v/v. The final residual glucose concentrations in the fermentation broth for vacuum and non-vacuum treatments were 1.5% and 0.1%, respectively, indicating a more complete fermentation with vacuum flash. However, ethanol yields for vacuum and non-vacuum treatments were similar, 0.288 ±0.013 L kg-1 and 0.285 ±0.013 L kg-1, respectively. Neither removal of CO2 nor repressurization with unﬁltered air affected ﬁnal ethanol yields.
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