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Effects of glucose, acetic acid and glycerol on biomass accumulation of Chlorella sp.

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  Paper number  131620778,  2013 Kansas City, Missouri, July 21 - July 24, 2013. (doi: http://dx.doi.org/10.13031/aim.20131620778) @2013
Authors:   F. Ji, Y. Liu, G. Li, Y. Zhou, L. Tian, Z. Ma, R. Dong
Keywords:   Chlorella sp., glucose, acetic acid, glycerol, response surface methodology.

Abstract. Biomass production by microalgae growth under mixotrophic condition, and mutual effects of glucose, acetic acid and glycerol on different organic carbon concentrations were studied in current research. The organic carbon concentrations for the enhancement of growth performance was optimized using response surface methodology. The maximum Chlorella sp. biomass of 0.724 g/L, 0.456 g/L and 0.405 g/L was observed within 5 days in the different media including glucose (30.0 g/L), acetic acid (3.0 g/L) and glycerol (5.0 g/L), respectively.

It indicated that the suitability of second-order polynomials could predict the response function. The maximum dry cell weight (0.547 g/L) of glucose, acetic acid and glycerol concentrations were 0.5 g/L, 0.5 g/L and 1.875 g/L with low organic carbon concentration, and 60.0 g/L, 1.0 g/L and 1.0 g/L with high organic carbon concentration, respectively.

The results indicated that biomass production of Chlorella sp. depends on the concentration of organic carbon source and chemical composition of organic matters. High concentrations of organic carbon substrate might lead to the inhibition. The mutual effect of organic carbon condition is also not benefit to the biomass accumulation of Chlorella sp., comparing with single organic carbon substrate.

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