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Development of rapid diagnosis technology for pig disease

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  Paper number  131577119,  2013 Kansas City, Missouri, July 21 - July 24, 2013. (doi: http://dx.doi.org/10.13031/aim.20131577119) @2013
Authors:   H J Kim, J T Hong, B K Yoo, Giyoung Kim, S Kim
Keywords:   Key Words: Porcine proliferative enteropathy (PPE) rapid diagnosis pig feces Lawsonia

Abstract: Porcine proliferative enteropathy (PPE), caused by the obligate intracellular bacterium Lawsonia intracellularis, is a widely distributed disease throughout the world causing substantial economic loss. The bacterial pathogen invades the intestinal epithelial cells which causes hyperplasia of the infected cells and leads to the process of disease pathogenesis. For diagnosing PPE, normally PCR test, fluorescence antibody test, histoimmunochemistry test method is used in the Lab, which takes 2~3 days or more to get the confirmation of the diagnosis for farmers. Also, farmers have to pay much money for these tests. To deal with the PPE effectively, it will be better for farmers to screen the PPE in earlier stage with easy and rapid diagnosing tool on farm.

In this study, for screening the PPE rapidly on farm with the developed rapid kit, we tested fecal samples of PPE infected pigs diagnosed by PCR method . As a result of this study, we present some results demonstrating that the kit could detect the Lawsonia in pig feces, and easy to use for PPE screening. But, from the rapid kit test result, we found that the non specific response to negative samples of for PPE is too high to use in the field. Therefore, we recommend that further research is needed for searching the optimum conditions for making the rapid kit to achieve very low non-specific response

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