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Determination of Degree of Infestation of Triticale Seed Using NIR Spectroscopy

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  Paper number  131592957,  2013 Kansas City, Missouri, July 21 - July 24, 2013. (doi: @2013
Authors:   Mahmoud K Khedher Agha, Won Suk (Daniel) Lee, Chuan Wang , Richard W Mankin, Nikolay Bliznyuk, , Ray A Bucklin
Keywords:   Rice Weevil Spectral reflectance stepwise multiple linear regression (SMLR).

Abstract. Insect infestation of triticale (Triticosecale) seed causes extraordinary storage losses as a consequence of vulnerability of triticale seed to insect infestation and its soft coat. Rice weevil, Sitophilus Oryzae (L.), is a common insect that causes infestation in Florida, which was the focus of this research. The objective of this research was to develop a method to determine the degrees of infestation (DI) in the seed at two growth stages by measuring their spectral reflectance. To achieve this goal, triticale samples with eleven DI (0, 6, 11… and 62%) were prepared with two growth stages of larvae 2nd instar, and adult outside seed with three to four replications. The reflectance was measured from 400 nm to 2500 nm. The DI results were compared to manual inspection in order to evaluate the accuracy. The data was analyzed using stepwise multiple linear regression (SMLR). The result showed that the DI for larvae 2nd instar stage could be detected using an average reflectance in 400 - 410 nm, with an R2 of 0.87 using the SMLR method. The adult outside stage also resulted in a good prediction using the SMLR method, where it yielded four wavelengths (400, 783, 791, and 967 nm) that provided an acceptable result with an R2 of 0.87 for the adult outside stage. The prediction of early growth stages was more challenging than for late growth stages, due to the smaller size of larvae 2nd instar stage compared to the adult stage. Overall, NIR spectroscopy was proved to be useful to detect insect infestation in triticale seed.

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