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Development of a disposable impedance biosensor and its application for determination of Escherichia coli O157:H7

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  Paper number  131593501,  2013 Kansas City, Missouri, July 21 - July 24, 2013. (doi: @2013
Authors:   Yixian Wang, Zunzhong Ye, Yibin Ying
Keywords:   Disposable biosensor Impedance Escherichia coli O157:H7 Graphene Gold nanoparticles

Abstract.The development of cost-effective, robust, and rugged biosensing devices is of great interest for point-of-care testing and on-spot monitoring. Here we reported a facile approach to build high-performance disposable impedance biosensor for sensitive and selective determination of Escherichia coli O157:H7. Gold nanoparticles and graphene modified screen-printed carbon electrode was employed as the electrode substrate in this impedance biosensor. The immobilization of anti-Escherichia coli O157:H7 antibodies on electrode surface were performed by simple physical adsorption. Electrochemical impedance spectroscopy was used to detect Escherichia coli O157:H7 captured on the disposable biosensor. Results show that the developed immunosensor possesses greatly enhanced sensing performance, such as wide linear range (1.5 × 103 - 1.5 × 107 cfu mL−1), low detection limit (1.5 × 103 cfu mL−1), and excellent specificity. The strategy of structurally integrating metal nanomaterials, graphene, and screen-printed electrode would provide new insight into design of disposable and high-performance immunosensors for routine sensing applications.

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