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Two-thirds of Alabama is covered with forest, and Alabama also ranks as the third largest state in the U.S. for poultry production. As a result, the state has significant amounts of forest residues and poultry litter available, which can be converted into biofuels. Bio-oil derived from pine wood is acidic, whereas bio-oil obtained from poultry litter is basic. The main objective of this work was to co-process woody biomass and poultry litter so that high pH bio-oil could be produced. Three different mixtures of poultry litter (5, 10, and 15 wt%) in pine wood were used to determine the effect of poultry litter on bio-oil yield and properties at 500C using an auger reactor. The pH of the bio-oil increased from 3.73 to 3.94 when 15 wt% of poultry litter was mixed with pine. However, the bio-oil yield decreased with the increase in poultry litter with pine wood. For example, at 5 wt% of poultry litter, the bio-oil yield was 37.3 wt%; however, at 15 wt% of poultry litter, the bio-oil yield was 34.8 wt%. This study concludes that the pH of bio-oil increased with the increase in poultry litter in the feedstock.