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Animal husbandry is a major source of atmospheric emissions, e.g., ammonia, and methane. The quantification of emission flow has gained special attention in the recent years. But, in naturally-ventilated barns this quantification is still a difficult task. A main issue is to estimate the air exchange rate (AER) of the barn, and then to quantify the emission flow. This study consisted of several trials conducted during mild and cold periods of the year in two naturally-ventilated dairy cattle barns located in northeast Germany. During the experiments, the concentrations of (NH3, CH4 and CO2) as well as the temperature were measured outside and inside the barns. The wind speed and the wind direction outside the barns were recorded as well as the air speed through the openings of the barns. The AERs were estimated according to three methods: concentration decay of radioactive isotope tracer-gas Krypton85 (85Kr-M), the air speed through the inlet openings (AS-M), and the CO2 mass balance model (CO2-M). The comparison was done by performing the Pearson correlation analysis and by developing a linear regression model.