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Dehumidification is essential for greenhouse humidity control due to the serious effect of high relative humidity on crops. A few dehumidification methods have been tested in a greenhouse. Mechanical refrigeration dehumidification is proved to be the most energy efficient and is the most promising method for greenhouses in cold regions. However, limited information exists on how to quantify the dehumidification requirement of a greenhouse. In this study, two methods, a moisture balance modeling method and an experimental measurement method, were employed to estimate the dehumidification needs of a commercial greenhouse. Experimental data collected from the same greenhouse with three dehumidification treatments, i.e. mechanical dehumidifiers, air-to-air heat exchangers, and temperature controlled ventilation with exhaust fans, were used to verify the predicted results of the two methods. The results revealed that compared with the moisture balance modeling, the experimental method was proved to be more accurate to estimate the greenhouse dehumidification. A more complete moisture balance model should be developed in the future.