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Polysaccharide Extracts from Lentinus Edodes Stems Assisted By High Pressures Processing

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  2012 Dallas, Texas, July 29 - August 1, 2012  121341142.(doi:10.13031/2013.42065)
Authors:   Jing Wang, Yong Yu, Jinsong He, Songming Zhu, Jianping Li
Keywords:   Polysaccharide, Lentinus edodes stems, High pressures processing, Extract

In this study, high pressure processing (HPP) was applied to extract polysaccharide from Lentinus edodes stems (LES) at ambient temperature. The effects of pressure,solvent-to-solid ratio and holding time on the yield of polysaccharide were studied by quadratic rotation-orthogonal composite experimental design. The linear effect of solvent-to-solid ratio was significant (p < 0.01). The interaction effect of pressure x holding time was significant (p < 0.05). The quadratic effects of pressure and solvent-to-solid ratio were significant (p < 0.05, p < 0.01, respectively). The optimal condition was: pressure at 210 MPa, solvent-to-solid ratio at 26 mL per 1g and holding time at 20 min, and the maximum yield of polysaccharide was 53.62 mg/g. Comparing to the conventional extraction method, HPP shows dominant advantages with higher yield and shorter extraction time. The use of HPP to extract polysaccharide from LES is a potential alternative to conventional methods.

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