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Presence of trash in commercial cotton bales compromises their market values and further influences the end-use qualities. In order to ensure a fair trading, the USDAs Agricultural Marketing Service (AMS) has implemented the high volume instrument (HVI) readings as the universal quality indices to grade the cottons. Compare to HVIs geometric method that represents the trash portion only on a samples surface, traditional Shirley Analyzer (SA) is a gravimetric-based method and is being routinely utilized in the laboratories. With the increasing acceptance of HVI readings in domestic and international trading, there is a continued interest in understanding the conversion constant between two trash testing results from the customers, regulatory and trading organizations. Due to the complexity of trash type and size and also the nature of HVI and SA measurement, apparently bridging the two types of trash readings is a great challenge. This investigation addressed the need by proposing an innovative approach to establish the conversion constant, through sub-grouping the samples and then verifying the findings from independent NIR technique.