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Detection of Airborne Salmonella spp. in Poultry Farms Using Impingement: Culture-Dependent vs. Culture-Independent Methods

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  2012 IX International Livestock Environment Symposium (ILES IX)  ILES12-1300.(doi:10.13031/2013.41559)
Authors:   Elisa Adell, Verónica Moset, Yang Zhao, Ana Jiménez-Belenguer, Alba Cerisuelo, María Cambra-López
Keywords:   Air quality, airborne pathogens, bioaerosols, broilers

Presence of airborne Salmonella species (spp). in poultry houses can pose a risk of infection to human and animal through airborne transmission route. The objective of this study was to assess the performance of impingement technique, followed by culture-dependent and culture-independent methods to detect airborne Salmonella spp. in rooms with experimentally inoculated birds. Broilers were inoculated at 7 days of age with a marked strain of Salmonella enteritidis. The rearing cycle lasted 42 days over the summer. Airborne Salmonella spp. was weekly sampled in each room with all-glass impingers (AGI-30). Presence of Salmonella spp. was determined by three culture-dependent methods (dilutions and plating, Most Probable Number-MPN and ISO 6579:2002); and by one culture-independent method (polymerase chain reaction, PCR). Additionally, Salmonella spp. was sampled on feeders, drinkers, walls, and in the litter. No cultivable Salmonella spp. on room floor and wall, feed or litter was recovered before bird inoculation. Salmonella spp. was detected on room surfaces after inoculation, and quantified in the litter in both rooms thereafter.

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