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High levels of relative humidity (RH) can easily occur inside greenhouses. It can lead to dew or water condensation on plant leaf surfaces; and condensation on the interior surface of covering materials also drips on plants, providing an ideal growing condition for fungal diseases. Limited information exists on how to quantify the dehumidification requirement of a greenhouse. In this study, a dehumidification method using an air-to-air heat exchanger was used to estimate the dehumidification need of a commercial greenhouse in a cold region. The experiment was conducted for 7 months and the greenhouse climatic parameters were monitored. The results showed that the heat exchanger controlled the RH very well in cold and mild seasons but its capacity was insufficient in summer time. The actual amount of moisture removed by the heat exchanger was calculated. Based on the experimental analysis, the dehumidification requirement for satisfactory control of humidity for the greenhouse was estimated.