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A Scale Up and Operation Problems for a Liquid Bioreactor of an Edible Fungus (Mushroom Agaricus blazei Murill.)

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  Paper number  016156,  2001 ASAE Annual Meeting. (doi: 10.13031/2013.4057) @2001
Authors:   Keo INTABON, Takaaki MAEKAWA, Norio SUGIURA
Keywords:   Agaricus blazei Murill mushroom, Liquid culture, Bacillus species,Contamination, Spawn

Bacillus species was the most critical contaminant for our liquid bioreactor in operation to produce filamentous mycelium of Agaricus blazei Murill. The bacterium was present in level of 10 5 in the autoclaved culture medium just before seeding. Some lots fertilized with 4L of spawn having mycelium biomass of about 4.752g/L were contaminated. Contamination risk was overcome however by doubling the spawn volume. The relationship between Bacillus count and growth behavior of 3 strains of filamentous mycelia used in laboratory cultures was described.

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