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Particle shape is increasingly important as a quality parameter for comminuted biomass. Shape affects flowability, pretreatment, rate of conversion, and performance of materials handling systems. This protocol applies to materials that have been subjected to commutation processes including but not limited to chunking, chipping, grinding or milling. Our objective is to enable a uniform terminology and method for characterizing particle shape of chipped, ground, or otherwise comminuted woody biomass. The protocol was specifically developed for chipped and ground woody biomass and may be applicable to other lignocellulosic raw materials and feedstocks. Results are presented for analysis of chipped and shredded forest derived woody biomass.