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Because of the highly consumed fossil fuels, it is an urgent affair to search for a renewable energy. Using biomass wastes as energy source to produce bio-ethanol not only reduces the demand of petroleum but prevents the competition between crop and energy crop plantation. Furthermore, it helps to diminish the amount of lignocellulosic biomass wastes such as agriculture and forest wastes and could reduce the greenhouse gas from fossil fuels. According to the prior art, cellulose could be digested using Clostridium cellulolyticum. In the future, C. cellulolyticum will be used as cultures in the fermentation test of Ma bamboo and an anaerobic reactor will be setup.