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Local runoff from the catchments in the Mount Lofty Ranges watershed provides a major source (up to 60%) of drinking water for Adelaide, South Australia. In this study major land uses in the Mount Lofty Ranges were monitored for off-site transport of sediment and nutrients over approximately 40 months. Runoff from three case-study sites (apple, cherry and grape cultivation) was monitored using automatic samplers for collection of water samples on a flow-weighted basis. Selected nutrients and the total suspended sediment (TSS) were measured in unfiltered and filtered water samples to provide information about pollutant characterization and the mode of off-site transport. Flow meters were installed at each site to measure runoff and enabled concentration loads to be calculated.