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The persistence and long life expectancy of ammonia, odors and toxic pollutants from poultry houses may be due to the ability of suspended particulate matters (SPM) to serve as carriers for odorous compounds such as ammonium ions and volatile organic compounds. SPM is generated from the feed, animal manure, and the birds themselves. A large portion of odor associated with exhaust air from poultry houses is SPM that have absorbed odors from within the houses. Understanding the fate and transport processes of particulate emissions in poultry house is a necessary first step in utilizing the appropriate abatement strategies. In this study, the examination of the effect of management strategies, in particular the feeding schedule, on the particulate matter (PM) distribution in a rooster house was carried out. Fractionated SPM (2.5 m, 10.0 m, and total inhalable fraction or TIH) were collected from a rooster house using particle trap impactors. The results showed that feeding regimen had a major effect on the SPM distribution in the poultry house. A marked increase in SPM concentrations was observed during non-feeding periods used to increase leanness in birds. During the 25 weeks of growth, the average concentration differences between feeding and non-feeding periods for PM2.5, PM10, and TIH were 58%, 104%, and 149%, respectively.