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Magnetic nanoparticles based magnetophresis for efficient separation of foodborne pathogens

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010  1009458.(doi:10.13031/2013.29912)
Authors:   Haibo Huang, Yanbin Li, Chuanmin Ruan, Jianhan Lin, Min Li, Lisa Cooney, Andrew Wang
Keywords:   Magnetophresis separator; Magnetic nanoparticles; Foodborne pathogens; High gradient magnetic separation

In this research, a magnetophoretic separation device was designed, fabricated and tested using magnetic nanoparticles (MNPs) for more efficiently separating target foodborne pathogens. This magnetophoretic separation device consisted of a rectangular channel with a steel rod on its top, both of which were immersed in a homogenous magnetic field generated by two permanent magnets. A sample containing foodborne pathogens was incubated with biotin conjugated antibody (Ab) and then streptavidin coated MNPs with a diameter of 30 nm to form MNPs-Ab-cell complexes, the magnetic cell. The magnetized steel rod generated a high magnetic field gradient, which could separate the magnetic cells from a background fluid without a need of repeated washing. Firstly, a mathematical model was developed in order to understand and predict the performance of this separation device. Secondly, the magnetophoretic separation device was tested experimentally using Escherichia coli O157:H7 as a model bacterium. The results showed that 97.4% of the E. coli O157: H7 cells could be separated from a phosphate buffered saline (PBS) solution, at a separation flow rate of 15 L/min. Also, a separation efficiency of 85.6% was achieved during the separation of E. coli O157:H7 from a mixture of E. coli O157:H7 and Listeria innocua in a PBS solution. The magnetophoretic separation device was shown to have potential for applications in the rapid detection of foodborne pathogens with improved sensitivity.

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