Click on “Download PDF” for the PDF version or on the title for the HTML version.

If you are not an ASABE member or if your employer has not arranged for access to the full-text, Click here for options.

Magnetic nanoparticles based magnetophresis for efficient separation of foodborne pathogens

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010  1009458.(doi:10.13031/2013.29912)
Authors:   Haibo Huang, Yanbin Li, Chuanmin Ruan, Jianhan Lin, Min Li, Lisa Cooney, Andrew Wang
Keywords:   Magnetophresis separator; Magnetic nanoparticles; Foodborne pathogens; High gradient magnetic separation

In this research, a magnetophoretic separation device was designed, fabricated and tested using magnetic nanoparticles (MNPs) for more efficiently separating target foodborne pathogens. This magnetophoretic separation device consisted of a rectangular channel with a steel rod on its top, both of which were immersed in a homogenous magnetic field generated by two permanent magnets. A sample containing foodborne pathogens was incubated with biotin conjugated antibody (Ab) and then streptavidin coated MNPs with a diameter of 30 nm to form MNPs-Ab-cell complexes, the magnetic cell. The magnetized steel rod generated a high magnetic field gradient, which could separate the magnetic cells from a background fluid without a need of repeated washing. Firstly, a mathematical model was developed in order to understand and predict the performance of this separation device. Secondly, the magnetophoretic separation device was tested experimentally using Escherichia coli O157:H7 as a model bacterium. The results showed that 97.4% of the E. coli O157: H7 cells could be separated from a phosphate buffered saline (PBS) solution, at a separation flow rate of 15 L/min. Also, a separation efficiency of 85.6% was achieved during the separation of E. coli O157:H7 from a mixture of E. coli O157:H7 and Listeria innocua in a PBS solution. The magnetophoretic separation device was shown to have potential for applications in the rapid detection of foodborne pathogens with improved sensitivity.

(Download PDF)    (Export to EndNotes)