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Optical Characterization of Biological Material: A Multiscale Approach

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010  1000032.(doi:10.13031/2013.29878)
Authors:   Wouter Saeys, Nghia Nguyen Do Trong, Rodrigo Watté, Mizuki Tsuta, Herman Ramon, Bart M Nicolaï
Keywords:   Optical properties, spatially resolved spectroscopy, microstructure, light propagation, Monte Carlo simulation.

The change of Vis/NIR radiation when propagating through biological material is the result of a complex process of molecule-specific absorptions and multiple light scattering caused by the interaction of the photons with the microstructure. In addition, many biological products, such as fruit or skin tissue are characterized by a layered structure. Therefore, multiple measurements are needed to separate the information on the different layers. In this research, reflectance measurements at different distances from the incident light beam are combined with multiscale light propagation models to extract the compositional and microstructure properties of biological products. First, the biological material is modelled at the macroscale (~mm) as a set of uniform layers, where the light propagation in each layer is defined by 3 bulk optical properties. Using these 3 bulk properties the reflectance spectra at different distances from the incident light beam are calculated by Monte Carlo simulations for the radiative transport equation.

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