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Quantification of Casein and Cellulose Binding Affinity

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010  1008882.(doi:10.13031/2013.29748)
Authors:   Dana Meredith Mears, Jeffrey Catchmark
Keywords:   .

Caseins, proteins in the milk of mammalian species, have been used as wood adhesives since the early 1900's. Caseins' ability to bind certain materials has been exploited in a variety of products over the years. However, the nature of the binding interaction remains unknown, and studies performed involve the collective casein system, or some of the isolated fractions, but not all of the individual or combinations of various casein subunits. The proposed research includes the separation and purification of the four casein subunits: alphas1-, alphas2-, beta, and kappa, by a simplified reverse phase high performance liquid chromatography (RP-HPLC) method. The binding affinity of the collective casein system, as well as the purified subunits, for various forms of cellulose is examined.

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