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Hydrothermal Pretreatment and Enzymatic Hydrolysis of Prairie Cord Grass

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010  1008844.(doi:10.13031/2013.29737)
Authors:   Iwona Cybulska, Hanwu Lei, James L Julson
Keywords:   Hydrothermal pretreatment, prairie cord grass, enzymatic hydrolysis, SEM.

Abstract The study focus was examination of the hydrothermal pretreatment method applied to the lignocellulosic substrate for bioethanol production, represented by prairie cord grass, and comparison between different conditions based on the yield of glucose after enzymatic hydrolysis. The treatment did not involve any chemicals usage. Hydrothermal treatment was conducted in the Parr high pressure reactor, in the presence of water. After the treatment, material was analyzed with HPLC for products and SEM for biomass structure changes. Enzymatic hydrolysis was performed in order to examine the amount of glucose which was released from pretreated materials. Results were compared also considering releasing of other sugars as well as generation of by-products. The final comparison was made based on conversion rates. Final results suggested that the most efficient pretreatment conditions were with high temperature and relatively short reaction time (210oC and 10 min), after which the lignocellulose structure seems to be the most available for enzymes actions. The pretreatment conversion rate in this case reached the level of 97%.

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