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Mathematic modeling of Rennin Production by Mucor miehei

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010  1008700.(doi:10.13031/2013.29708)
Authors:   Yang Hu, Gulten Izmirlioglu, Dana Mears-Leiner, Evan Rusadi
Keywords:   Mucor miehei, Rennin, Fermentation, Modeling.

Rennin is the most popular enzyme in the food industry as a coagulator of milk to produce cheese. An efficient large-scale fermentation for rennin production by Mucor miehei is developed by the mathematic equations to combat the need for rennin not derived from infant ruminants. Scaling-up of the fermentation process, involving calculations to derive industrial parameters from the laboratory are used extensively to determine the feasibility of the developed process. Limitations and recommendations of the design are discussed to point out the complications of assumptions and to grasp engineering ingenuity by circumnavigating such problems. Ultimately we show that using aerobic liquid fermentation in a continuously fed fermenter, 1,580,000 grams of rennin per annum (an estimated 10% of market supply) can be produced with three 80,000 liter bioreactors after inoculation with 5.2% (v/v) M. miehei. After the fermentation process, important rennin separation and purification processes are discussed: filtration, ammonium sulfate precipitation, anion-exchange chromatography and crystallization.

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