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Modeling of Pullulan Fermentation by Using a Color Variant Strain of Aureobasidium pullulans

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010  1008676.(doi:10.13031/2013.29695)
Authors:   Kuan-Chen Cheng, Ali Demirci, Jeffrey M Catchmark, Virendra M Puri
Keywords:   Aureobasidium pullulans, pullulan, Logistic model, Leudeking-Piret model, Gompertz model.

Pullulan, which is comprised of glucose units, is a simple linear polysaccharide produced by Aureobasidium pullulans. Pullulan has long been used in various applications such as blood plasma substitutes, food additives, adhesive additives, flocculants, and even environmental pollution control agents. Mathematical models of biomass, pullulan, and sucrose profiles during fermentation not only provide information about the kinetic-metabolic nature of pullulan, but also facilitate the control and optimization of pullulan production. In this study, several models were modified and tested in order to describe biomass, pullulan, and sucrose profiles during batch fermentation using a color variant strain of A. pullulans. The results demonstrated that the modified Gompertz model can serve as a generalized equation to fit biomass production, pullulan production, and sucrose consumption. Furthermore, validation of this modified Gompertz model indicated that biomass (slope = 1.00, R2 = 0.991), pullulan (slope = 1.10, R2 = 0.991), and sucrose (slope = 0.96, R2 = 0.991) were all predicted accurately.

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