Click on “Download PDF” for the PDF version or on the title for the HTML version.

If you are not an ASABE member or if your employer has not arranged for access to the full-text, Click here for options.


Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010  1008672.(doi:10.13031/2013.29691)
Authors:   Gulten Izmirlioglu, Ali Demirci
Keywords:   Enzyme hydrolysis, waste potato, liquefaction, saccharification, response surface method.

The potato is a starchy, tuberous crop vegetable which is one of the carbon sources for ethanol fermentation. However, a pretreatment process is needed to convert starch to fermentable carbon sources, glucose. In order to obtain a maximum glucose conversion, relationship among parameters of the liquefaction and saccharification process were investigated by response surface method. A Box-Behnken Surface Response Method was performed to attain optimum conditionals for high conversion yield. The optimum combination of temperature, dose of enzyme (a-amylase), and amount of potato was 95C, 1 ml enzyme (18.8 mg protein/ml), and 4.04 g dry-weight/100 ml DI water, respectively with a 68.86% loss in dry weight. For saccharification, dose of enzyme, temperature, and saccharification time were examined by creating Box-Behnken Surface Response design and 60C-72 h-0.8 ml (300 Unit/ml) of amyloglucosidase combination was determined as optimum conditionals with 34.9 g/L glucose.

(Download PDF)    (Export to EndNotes)