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Removal of Phenolic Compounds by Adsorption on Polymeric Resins before Protein Purification

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010  1008616.(doi:10.13031/2013.29671)
Authors:   Georgia O. F Barros, Susan L Woodard, Zivko L Nikolov
Keywords:   Transgenic plants, downstream processing, phenolic compounds, adsorption isotherms, ferulic acid, chlorogenic acid, syringic acid, rutin, vitexin-2-O-rhamnoside

Transgenic plant systems have been proposed as alternative bioreactors to mammalian cell culture in the production of a variety of pharmaceutical proteins and other bioproducts. Phenolic compounds in transgenic plant extracts present obstacles in the downstream processes development since they can interact with the target proteins, and foul membranes and chromatographic resins during purification. Because phenolics comprise a large variety of compounds, different methods can be applied for their removal, such as solid-liquid adsorption, aqueous liquid-liquid extraction, precipitation, etc. Polymeric resins have also been proven to be a desirable pretreatment option for processing extracts from plants that have been modified to produce recombinant proteins. Each plant system has a unique composition of phenolic compounds which may interact in different ways with the polymeric resins. Understanding phenolics-resin interactions would allow the design of an optimal extract pretreatment by selecting the appropriate resin for phenolics present in the plant extract. Solutions of standard phenolic compounds (chlorogenic acid, ferulic acid, rutin, syringic acid and vitexin-2-O-rhamnoside) from different phenolic compound classes (hydroxycinnamic acids, hydroxybenzoic acids, flanovols, and flavones) were studied, and adsorption isotherms of these compounds with three commercial resins (IRA-402, PVPP, XAD-4) at pH 4.5 and 7.5 were determined.

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