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Surface-enhanced Raman Scattering Spectroscopy for Rapid Bacterial Screening

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010  1008542.(doi:10.13031/2013.29643)
Authors:   Kuanglin Chao, Moon S Kim, Chun-Chieh Yang, Jianwei Qin, Yongliang Liu
Keywords:   SERS, Silver colloid, E coli, Listeria, Salmonella, Food safety

This study reports the feasibility of citrate-reduced colloidal silver SERS for differentiating three important foodborne pathogens, E. coli, Listeria, and Salmonella. FT-Raman and SERS spectra of both silver colloids and colloid-K3PO4 mixtures were collected and analyzed to evaluate the reproducibility and stability of silver colloids fabricated in a batch-production process. The results suggest that the reproducibility of the colloids over the batch process is high and that their binding effectiveness remains consistent over a 60-day storage period. Two specific SERS bands at 712 and 390 cm-1 were identified and used to develop simple 2-band ratios for differentiating E. coli-, Listeria-, and Salmonella-colloid mixtures with a 100% success. These results indicate that colloidal silver SERS technique may be a practical alternative method suitable for routine and rapid screening of E. coli, Listeria, and Salmonella bacteria.

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