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Anthropogenic Influence on Tetracycline Resistance in a Subtropical Watershed

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  21st Century Watershed Technology: Improving Water Quality and Environment Conference Proceedings, 21-24 February 2010, Universidad EARTH, Costa Rica  701P0210cd.(doi:10.13031/2013.29398)
Authors:   Bailey Ann Sullivan, Raghupathy Karthikeyan
Keywords:   antibiotic resistant genes, water quality, PCR

The ability of antibiotics to prevent disease and death has deteriorated due to the development of antibiotic resistan species. Antibiotic resistance has been shown to be caused by misuse of antibiotics in both human and agricultural use. The overall objective of this research is to determine the anthropogenic influences on the development of tetracycline resistant genes and to aid in the development of best management practices to minimize the transport of tetracycline resistant genes in the environment. This study will determine if tetracycline resistant genes are present in subtropical watershed which is a located in E.C. Texas. Five sites with different land uses along the stream were sampled and the presence or absence of 3 tetracycline resistant genes (tet(O), tet(W), tet (Q)) was determined using PCR, also the samples were plated on nutrient agar plates containing three treatments of tetracycline ( 0 mg/L, 14 mg/L, and 28 mg/L). Samples were taken five times during the summer/spring season and winter/fall. The data was analyzed using SAS to determine if there is a significant difference between land use, seasonality, and media. All five sites had positive growth on the mild tetracycline treatment and a majority of the sites tested positive at the high tetracycline treatment, indicating that antibiotic resistance is a problem for this watershed.

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